He was born in Koper on 29 March 1561 to Antonio, originally from Friuli, and Elisabetta Cordonia, from Koper. Antonio, the father, served the Serenissima as ammunition superintendent and at an early age Santorio moved to the lagoon city together with his brother Isidoro, while his sisters Piana and Franceschina remained in Koper where they married.
In Venice he was able to study the humanities thanks to the support of the Morisini patricians, friends of the Sartorio family, and in 1575 he studied philosophy with Giacomo Zabarella and medicine with Bernardino Paterno; during these
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